ATRIP Antibody

Immunoprecipitation: ATRIP Antibody [NB100-359] - Whole cell lysate from HeLa and 293-T cells. ATRIP was also IPed using a control antibody generated to recombinant human ATRIP. IPed ATRIP was detected using the antibody to the recombinant protein.

Product Discontinued

ATRIP Antibody Summary

• Immunogen: A synthetic peptide derived from a sequence between amino acids 500-550 of human ATRIP [UniProt# Q8WXE1]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATRIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 3 -8 ug/mg of lysate
  • Western Blot 1:5000-1:15000
• Application Notes: This ATRIP antibody is useful for Western blot and Immunoprecipitation. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  ATRIP Overexpression Lysate

Reactivity Notes

Human. Reactivity with intraprimates is unlikely owing to immunogen sequence divergence.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATRIP Antibody

• AGS1
• ATM and Rad3-related-interacting protein
• ATR interacting protein
• ATR-interacting protein
• ATRIP
• DKFZp762J2115
• FLJ12343
• MGC20625
• MGC21482
• MGC26740
• TREX1

Background

ATRIP is an 86 kDa protein that is phosphorylated by ATR, regulates ATR expression, and is an essential component of the DNA damage checkpoint pathway. It localizes to the intranuclear foci after DNA damage or inhibition of replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATRIP Antibody (NB100-359)(1)

Publication using NB100-359

• Choi, J et al. Reconstitution of a human ATR-mediated checkpoint response to damaged DNA. 104(33): 13301-13306. 2007-01-01 [PMID: 17686975]

Bioinformatics

• Gene Symbol: ATRIP
• Entrez:
  • 84126(Human)
• Uniprot:
  • Q8WXE1()