ATR Antibody

Images

 
Western Blot: ATR Antibody [NBP1-77232] - K562 cell lysates with ATR antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: ATR Antibody [NBP1-77232] - K562 cells with ATR antibody at 2 ug/ml.
Immunofluorescence: ATR Antibody [NBP1-77232] - Immunofluorescence of ATR in K562 cells with ATR antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ATR Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 13 amino acids near the C-terminus of human ATR. The immunogen is located within the last 50 amino acids of ATR.
Specificity
ATR antibody will recognize only the largest isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATR
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunocytochemistry
  • Immunofluorescence
Application Notes
ATR antibody can be used for detection of ATR by Western blot at 1 to 2 mg/ml. ATR will recognize only the largest isoform.
Control Peptide
ATR Peptide (NBP1-77232PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for ATR Antibody

  • ataxia telangiectasia and Rad3 related
  • Ataxia telangiectasia and Rad3-related protein
  • ATR
  • EC 2.7.11.1
  • FRAP-related protein 1
  • FRP1
  • FRP1FRAP-related protein-1
  • MEC1
  • protein kinase ATR
  • Rad3 related protein
  • SCKL1
  • SCKLMEC1, mitosis entry checkpoint 1, homolog
  • serine/threonine-protein kinase ATR

Background

The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ATR Antibody (NBP1-77232)

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Bioinformatics

Gene Symbol ATR
Entrez
Uniprot