ATPG Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-92867] - Paraffin-embedded rat ovary using ATPG .

Immunocytochemistry/Immunofluorescence: ATPG Antibody [NBP2-92867] - Analysis of U-2 OS cells using ATPG . Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: ATPG Antibody [NBP2-92867] - Analysis of C6 cells using ATPG . Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: ATPG Antibody [NBP2-92867] - Analysis of L929 cells using ATPG . Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-92867] - Paraffin-embedded human lung cancer using ATPG .

Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-92867] - Paraffin-embedded human placenta using ATPG .

Western Blot: ATPG Antibody - Azide and BSA Free [NBP2-92867] - Western blot analysis of various lysates, using ATPG Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATPG Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ATPG Antibody - Azide and BSA Free (NBP2-92867) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1). ATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARIYGLGSLALYEKADIKGPEDKKKHLLIGVSSDRGLCGAIHSSIAKQMKSEVATLTAAGKEVMLVGIGDKIRGILYRTHSDQFLVAFKEVGRKPPTFGDASVIALELLNSGYEFDEGSIIFNKFRSVISYKTEEKPIFSLNTVASADSMSIYDDIDADVLQNYQEYNLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5F1C
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:2000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for ATPG Antibody - Azide and BSA Free

• ATP synthase gamma chain, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• ATP5CATP synthase subunit gamma, mitochondrial
• ATP5CL1mitochondrial ATP synthase, gamma subunit 1
• F-ATPase gamma subunit

Background

ATP5C1( AAH00931, 1 a.a. - 299 a.a.) full-length recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ATP5F1C