ATPG Antibody - BSA Free

Western Blot: ATPG Antibody [NBP2-15525] - A: HeLa 12% SDS PAGE ATP synthase gamma(mitochondrial) antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-15525] - Paraffin-embedded mouse cervix. ATP synthase gamma antibody dilution: 1:500.

Western Blot: ATPG Antibody [NBP2-15525] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: ATPG Antibody [NBP2-15525] - A. 50 ug rat kidney lysate/extract 12% SDS-PAGE ATP synthase gamma antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-15525] - Paraffin-embedded mouse urinary bladder. ATP synthase gamma antibody dilution: 1:500.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATPG Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATPG. The exact sequence is proprietary.
• Localization: Mitochondrion; Mitochondrion inner membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5F1C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:1000-1:10000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ATPG Antibody - BSA Free

• ATP synthase gamma chain, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• ATP5CATP synthase subunit gamma, mitochondrial
• ATP5CL1mitochondrial ATP synthase, gamma subunit 1
• F-ATPase gamma subunit

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ATPG Antibody (NBP2-15525)(1)

Publication using NBP2-15525

• Song KH, Kim JH, Lee YH et al. Mitochondrial reprogramming via ATP5H loss promotes multimodal cancer therapy resistance J. Clin. Invest. 2018-08-31 [PMID: 30124467] (Mouse)

Bioinformatics

• Gene Symbol: ATP5F1C
• Uniprot:
  • P36542(Human)