ATPB Antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-ATP5B )

Product Discontinued

ATPB Antibody Summary

• Immunogen: The immunogen for this antibody was a sequence from 291a.a. to 539a.a. from ATP synthase beta(a 539a.a. long protein)
• Localization: Endoplasmic reticulum; endoplasmic reticulum lumen (By similarity). Mitochondrion. Mitochondrion inner membrane. Note=Peripheral membrane protein.
• Specificity: ATPB
• Clonality: Polyclonal
• Host: Chicken
• Gene: ATP5F1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:200-1:500
• Application Notes: Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
• Control:

  ATPB Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human, mouse, rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for ATPB Antibody

• ATP synthase subunit beta, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• ATPMB
• ATPSBmitochondrial ATP synthase beta subunit
• EC 3.6.3
• EC 3.6.3.14
• MGC5231
• mitochondrial ATP synthetase, beta subunit

Background

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1B
• Entrez:
  • 506(Human)
  • 11947(Mouse)
  • 171374(Rat)
• Uniprot:
  • P06576(Human)