ATPB Antibody (OTI4E5) - Azide and BSA Free

Western Blot: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATPB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATPB.

Immunocytochemistry/Immunofluorescence: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATPB.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded Ovary tissue using anti-ATPB mouse monoclonal antibody.

Flow Cytometry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Analysis of Jurkat cells, using anti-ATPB antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western Blot: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ATPB monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded breast tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded endometrium tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded liver tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded lung tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded pancreas tissue using anti-ATPB mouse monoclonal antibody.

Flow Cytometry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Analysis of Hela cells, using anti-ATPB antibody, (Red) compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI4E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ATPB Antibody (OTI4E5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ATPB Antibody (OTI4E5) - Azide and BSA Free (NBP2-02249) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP5F1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 56.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ATPB Antibody (OTI4E5) - Azide and BSA Free

• ATP synthase subunit beta, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• ATPMB
• ATPSBmitochondrial ATP synthase beta subunit
• EC 3.6.3
• EC 3.6.3.14
• MGC5231
• mitochondrial ATP synthetase, beta subunit

Background

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1B