ATP6AP1 Overexpression Lysate (Native)


Western Blot: ATP6AP1 Overexpression Lysate (Adult Normal) [NBL1-07831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATP6AP1.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

ATP6AP1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC200394 and based on accession number NM_001183. The protein contains a C-terminal DDK tag.
C-terminal Myc/DDK
Homo sapiens ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
51.8 kDa

Lysate Details for ATP6AP1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ATP6AP1 Overexpression Lysate (Native)

  • 16A
  • Ac45
  • ATP6IP1V-ATPase subunit S1
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1
  • ATPase, H+ transporting, lysosomal accessory protein 1
  • H+ transporting, lysosomal interacting protein 1
  • H-ATPase subunit
  • MGC129781
  • Protein XAP-3
  • Vacuolar proton pump subunit S1
  • VATPS1V-ATPase Ac45 subunit
  • V-type proton ATPase subunit S1
  • XAP-3
  • XAP3V-ATPase S1 accessory protein


This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Rt
Applications: IHC, ICC, ICFlow
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB, Flow, IHC
Species: Hu
Applications: WB, IP, PLA
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, IHC, IF
Species: Hu
Applications: WB
Species: Mu
Applications: WB, Simple Western
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for ATP6AP1 Lysate (NBL1-07831) (0)

There are no publications for ATP6AP1 Lysate (NBL1-07831).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ATP6AP1 Lysate (NBL1-07831) (0)

There are no reviews for ATP6AP1 Lysate (NBL1-07831). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATP6AP1 Lysate (NBL1-07831) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ATP6AP1 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for ATP6AP1 Lysate (NBL1-07831)

Discover related pathways, diseases and genes to ATP6AP1 Lysate (NBL1-07831). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATP6AP1 Lysate (NBL1-07831)

Discover more about diseases related to ATP6AP1 Lysate (NBL1-07831).

Pathways for ATP6AP1 Lysate (NBL1-07831)

View related products by pathway.

PTMs for ATP6AP1 Lysate (NBL1-07831)

Learn more about PTMs related to ATP6AP1 Lysate (NBL1-07831).

Blogs on ATP6AP1

There are no specific blogs for ATP6AP1, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol ATP6AP1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-07831 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

ATP6AP1 Recombinant Protein