ATP6AP1 Overexpression Lysate (Native)

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Western Blot: ATP6AP1 Overexpression Lysate (Adult Normal) [NBL1-07831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATP6AP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ATP6AP1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200394 and based on accession number NM_001183. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1), mRNA.
Gene
ATP6AP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
51.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ATP6AP1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ATP6AP1 Overexpression Lysate (Native)

  • 16A
  • Ac45
  • ATP6IP1V-ATPase subunit S1
  • ATP6S1ORF
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1
  • ATPase, H+ transporting, lysosomal accessory protein 1
  • H+ transporting, lysosomal interacting protein 1
  • H-ATPase subunit
  • MGC129781
  • Protein XAP-3
  • Vacuolar proton pump subunit S1
  • VATPS1V-ATPase Ac45 subunit
  • V-type proton ATPase subunit S1
  • XAP-3
  • XAP3V-ATPase S1 accessory protein

Background

This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ATP6AP1 Lysate (NBL1-07831) (0)

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Bioinformatics

Gene Symbol ATP6AP1