ATP5I Antibody

Images

 
Western Blot: ATP5I Antibody [NBP1-89496] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: ATP5I Antibody [NBP1-89496] - Staining of human cell line A-431 shows positivity in mitochondria.
Immunohistochemistry-Paraffin: ATP5I Antibody [NBP1-89496] - Staining of human stomach shows strong cytoplasmic positivity(granular pattern) in glandular cells.
Western Blot: ATP5I Antibody [NBP1-89496] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit

Order Details

ATP5I Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:RRIAAEEKKKQDELKRIARELAEDDSILK
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5I
Purity
Immunogen affinity purified
Innovator's Reward
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:250
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.
Control Peptide
ATP5I Protein (NBP1-89496PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (84%)

Alternate Names for ATP5I Antibody

  • ATP synthase e chain, mitochondrial
  • ATP synthase subunit e, mitochondrial
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E
  • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E
  • ATP5K
  • ATPase subunit e
  • F1F0-ATP synthase, murine e subunit
  • MGC12532

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATP5I Antibody (NBP1-89496) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol ATP5I

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