ATP5I Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ATP5I Antibody [NBP1-89496] - Staining of human cell line A-431 shows localization to mitochondria.Antibody staining is shown in green.

Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Staining of human testis shows strong cytoplasmic granular positivity in Leydig cells.

Staining of human duodenum shows strong cytoplasmic granular positivity in glandular cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Staining of human liver shows strong cytoplasmic granular positivity in hepatocytes.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ATP5I antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP5I Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATP5I Antibody - BSA Free (NBP1-89496) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RRIAAEEKKKQDELKRIARELAEDDSILK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5ME
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ATP5I Protein (NBP1-89496PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ATP5I Antibody - BSA Free

• ATP synthase e chain, mitochondrial
• ATP synthase subunit e, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E
• ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E
• ATP5K
• ATPase subunit e
• F1F0-ATP synthase, murine e subunit
• MGC12532

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the innermembrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalyticcore, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a singlerepresentative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodesthe e subunit of the F0 complex. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5ME