ATP5A Antibody (1082626) [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clone: 1082626
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

ATP5A Antibody (1082626) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects a synthetic peptide around aa 235 in Direct ELISA
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ATP5A Antibody (1082626) [Alexa Fluor™ Plus 488]

• ATP synthase alpha chain, mitochondrial
• ATP synthase subunit alpha, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 co
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2
• ATP sythase (F1-ATPase) alpha subunit
• ATP5A
• ATP5A1
• ATP5AL2
• ATP5AMOM2
• ATPM
• ATPMATP synthase subunit alpha, mitochondrial
• cardiac muscle
• COXPD22
• EC 3.6.3
• EC 3.6.3.14
• epididymis secretory sperm binding protein Li 123m
• hATP1
• HEL-S-123m
• MC5DN4
• MC5DN4A
• MC5DN4B
• mitochondrial ATP synthetase, oligomycin-resistant
• MOM2
• OMR
• ORM
• ORMATP synthase alpha chain, mitochondrial

Background

ATP5F1A is a 553aa, 60kDa protein which encodes subunit alpha of mitochondrial ATP synthase (complex V), the rotary ATPase responsible for more than 90% of cellular ATP synthesis.  F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c. ATP5A1 is generally regarded to mediate tumor progression through mitochondrial signaling.  It is overrepresented in an oxidative phosphorylation pathway associated with tumorigenesis and tumor progression. Dysregulation of this ATP synthase subunit is observed in human carcinomas.

1. Lines MA, Cuillerier A, Chakraborty P, Naas T, Duque Lasio ML, Michaud J, Pileggi C, Harper ME, Burelle Y, Toler TL, Sondheimer N, Crawford HP, Millan F, Geraghty MT. A recurrent de novo ATP5F1A substitution associated with neonatal complex V deficiency. Eur J Hum Genet. 2021 Nov;29(11):1719-1724. doi: 10.1038/s41431-021-00956-0. Epub 2021 Sep 6. PMID: 34483339; PMCID: PMC8560863.
2. Song Ba Y, Wang Ma F, Wei Ma Y, Chen Ba D, Deng Ba G. ATP5A1 Participates in Transcriptional and Posttranscriptional Regulation of Cancer-Associated Genes by Modulating Their Expression and Alternative Splicing Profiles in HeLa Cells. Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211039126. doi: 10.1177/15330338211039126. PMID: 34520292; PMCID: PMC8445539.
3. Yuan L, Chen L, Qian K, Wang G, Lu M, Qian G, Cao X, Jiang W, Xiao Y, Wang X. A novel correlation between ATP5A1 gene expression and progression of human clear cell renal cell carcinoma identified by co‑expression analysis. Oncol Rep. 2018 Feb;39(2):525-536. doi: 10.3892/or.2017.6132. Epub 2017 Dec 4. PMID: 29207195; PMCID: PMC5783621.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.