ATG7 Antibody

Product Discontinued

ATG7 Antibody Summary

• Immunogen: This affinity purified ATG7 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 550-575 of Human ATG7 protein. (Uniprot: O95352)
• Predicted Species: Human (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:300000-1:400000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in size corresponding to human Apg7 by western blotting in the appropriate cell lysate or extract. Human Apg7 has been reported to form a homodimer at approximately 160 kDa.
• Theoretical MW: 77.96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ATG7 Overexpression Lysate

• Control Peptide:

  ATG7 Protein (NBP1-96759)

Reactivity Notes

BLAST analysis indicates 100% homology (12/12) for this protein from chimpanzee, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D. melanogaster.
Mouse reactivity reported in scientific literature (PMID: 27551448).

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for ATG7 Antibody

• APG7 autophagy 7-like (S. cerevisiae)
• APG7
• APG7L
• APG7-LIKE
• ATG12-activating enzyme E1 ATG7
• ATG7 autophagy related 7 homolog (S. cerevisiae)
• ATG7
• Autophagy-related protein 7
• DKFZp434N0735
• GSA7
• hAGP7
• ubiquitin activating enzyme E1-like protein
• Ubiquitin-activating enzyme E1-like protein
• ubiquitin-like modifier-activating enzyme ATG7

Background

Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATG7 Antibody (NB600-1274)(4)

Publications using NB600-1274

• Rashid MM, Runci A, Polletta L et al. Muscle LIM protein/CSRP3: a mechanosensor with a role in autophagy. Cell Death Discov. [PMID: 27551448] (WB, Mouse)
• Lee J-A, Gao F-B. Inhibition of Autophagy Induction Delays Neuronal Cell Loss Caused by Dysfunctional ESCRT-III in Frontotemporal Dementia. J Neurosci. 2009-01-01 [PMID: 19571141]
• Razi M, Chan EYW, Tooze SA. Early endosomes endosomal coatomer are required for autophagy. J Cell Biol. 2009-01-01 [PMID: 19364919]
• Park,K et al. A Human scFv Antibody against TRAIL Receptor 2 Induces Autophagic Cell Death in Both TRAIL-Sensitive TRAIL-Resistant Cancer Cells. Cancer Res; 67: 7327 - 7334. [PMID: 17671202]

Bioinformatics

• Gene Symbol: ATG7
• Entrez:
  • 10533(Human)