ATG7 Antibody (SC06-30)

Western Blot: ATG7 Antibody (SC06-30) [NBP2-67596] - Analysis of Apg7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysatesLane 2: Jurkat cell lysates Lane 3: 293T cell lysates

Immunocytochemistry/Immunofluorescence: ATG7 Antibody (SC06-30) [NBP2-67596] - Staining Apg7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATG7 Antibody (SC06-30) [NBP2-67596] - Analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin.

Flow Cytometry: ATG7 Antibody (SC06-30) [NBP2-67596] - Analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ATG7 Antibody (SC06-30) [NBP2-67596] - Staining Apg7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATG7 Antibody (SC06-30) [NBP2-67596] - Analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATG7 Antibody (SC06-30) [NBP2-67596] - Analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATG7 Antibody (SC06-30) [NBP2-67596] - Analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin.

Product Discontinued

ATG7 Antibody (SC06-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ATG7 aa 654-703 / 703. (SwissProt: O95352 Human; SwissProt: Q9D906 Mouse)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATG7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ATG7 Antibody (SC06-30)

• APG7 autophagy 7-like (S. cerevisiae)
• APG7
• APG7L
• APG7-LIKE
• ATG12-activating enzyme E1 ATG7
• ATG7 autophagy related 7 homolog (S. cerevisiae)
• ATG7
• Autophagy-related protein 7
• DKFZp434N0735
• GSA7
• hAGP7
• ubiquitin activating enzyme E1-like protein
• Ubiquitin-activating enzyme E1-like protein
• ubiquitin-like modifier-activating enzyme ATG7

Background

FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATG7 Antibody (NBP2-67596)(1)

Publication using NBP2-67596

• Kim M, Chen C, Yaari Z et al. Nanosensor-based monitoring of autophagy-associated lysosomal acidification in vivo Nature chemical biology 2023-06-15 [PMID: 37322156]

Bioinformatics

• Gene Symbol: ATG7