ATG4D Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP1-33488] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded rat muscle. ATG4D antibody [N3C3] diluted at 1:500.

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded SAS xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded mouse ovary. ATG4D antibody [N3C3] diluted at 1:500.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATG4D Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATG4D Antibody - BSA Free (NBP1-33488) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (97%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG4D
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ATG4D Antibody - BSA Free

• APG4 autophagy 4 homolog D (S. cerevisiae)
• APG4 autophagy 4 homolog D
• APG4D
• APG4-D
• ATG4 autophagy related 4 homolog D (S. cerevisiae)
• AUTL4
• AUT-like 4 cysteine endopeptidase
• AUT-like 4, cysteine endopeptidase (S. cerevisiae)
• AUT-like 4, cysteine endopeptidase
• autophagin-4
• Autophagy-related cysteine endopeptidase 4
• Autophagy-related protein 4 homolog D
• cysteine protease ATG4D
• cysteine protease involved in autophagy
• EC 3.4.22
• EC 3.4.22.-

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.; FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide; SUBCELLULAR LOCATION: Cytoplasm; TISSUE SPECIFICITY: Mainly expressed in skeletal muscle and, to a lower extent, in testis.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4D
• Entrez:
  • 84971(Human)
  • 235040(Mouse)
  • 686505(Rat)
• Uniprot:
  • Q86TL0(Human)