ATG4D Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ATG4D Antibody [NBP1-33488] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200.Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded rat muscle. ATG4D antibody [N3C3] diluted at 1:500.
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded SAS xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded mouse ovary. ATG4D antibody [N3C3] diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Order Details

ATG4D Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Porcine (97%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG4D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATG4D Antibody

  • APG4 autophagy 4 homolog D (S. cerevisiae)
  • APG4 autophagy 4 homolog D
  • APG4D
  • APG4-D
  • ATG4 autophagy related 4 homolog D (S. cerevisiae)
  • AUTL4
  • AUT-like 4 cysteine endopeptidase
  • AUT-like 4, cysteine endopeptidase (S. cerevisiae)
  • AUT-like 4, cysteine endopeptidase
  • autophagin-4
  • Autophagy-related cysteine endopeptidase 4
  • Autophagy-related protein 4 homolog D
  • cysteine protease ATG4D
  • cysteine protease involved in autophagy
  • EC 3.4.22
  • EC 3.4.22.-

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.; FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide; SUBCELLULAR LOCATION: Cytoplasm; TISSUE SPECIFICITY: Mainly expressed in skeletal muscle and, to a lower extent, in testis.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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ATG4D NBP1-33488

Bioinformatics Tool for ATG4D Antibody (NBP1-33488)

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Diseases for ATG4D Antibody (NBP1-33488)

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Blogs on ATG4D.

ATG4D - A regulator of autophagy and apoptosis
Autophagy is an essential cellular process whereby damaged proteins and organelles are degraded and recycled. Autophagy, while happening constantly at a basal level, is tightly regulated and can be further induced under cellular stress. One of the...  Read full blog post.

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Bioinformatics

Gene Symbol ATG4D
Entrez
Uniprot