ATG4C Antibody

Product Discontinued

ATG4C Antibody Summary

• Immunogen: A synthetic peptide as a part of human, rat, mouse human, rat, mouse cysteine protease ATG4C (autophagin-3, APG4C, AUTL1, AUTL3) conjugated to immunogenic carrier protein has been used as the immunogen.
• Specificity: ATG4C.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ATG4C
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-10 ug/ml
• Control:

  ATG4C Overexpression Lysate

Reactivity Notes

Human, mouse and rat. Other species not yet tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: IgG purified
• Reconstitution Instructions: Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for ATG4C Antibody

• APG4 autophagy 4 homolog C (S. cerevisiae)
• APG4 autophagy 4 homolog C
• APG4C
• ATG4 autophagy related 4 homolog C (S. cerevisiae)
• AUT (S. cerevisiae)-like 1, cysteine endopeptidase; AUT-like 1, cysteineendopeptidase (S. cerevisiae)
• AUTL1
• AUTL3APG4-C
• AUT-like 1, cysteine endopeptidase
• AUT-like 3 cysteine endopeptidase
• autophagin-3
• Autophagy-related cysteine endopeptidase 3
• Autophagy-related protein 4 homolog C
• cysteine protease ATG4C
• EC 3.4.22
• EC 3.4.22.-
• FLJ14867

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide.; TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, liver and testis.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4C
• Entrez:
  • 84938(Human)
  • 242557(Mouse)
  • 313391(Rat)
• Uniprot:
  • Q96DT6(Human)