ATG4B Antibody [DyLight 594]

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 594

ATG4B Antibody [DyLight 594] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B.
• Specificity: At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG4B
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-41307. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for ATG4B Antibody [DyLight 594]

• APG4 autophagy 4 homolog B (S. cerevisiae)
• APG4 autophagy 4 homolog B
• Apg4B
• ATG4 autophagy related 4 homolog B (S. cerevisiae)
• ATG4B
• AUTL1
• AUTL1MGC1353
• AUT-like 1 cysteine endopeptidase
• autophagin-1
• Autophagy-related cysteine endopeptidase 1
• Autophagy-related protein 4 homolog B
• cysteine protease ATG4B
• DKFZp586D1822
• EC 3.4.22
• EC 3.4.22.-
• hAPG4B
• KIAA0943

Background

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4B