ATG16L1 Antibody

Western Blot: ATG16L1 Antibody [NBP2-24730] - Analysis of APG16L in human skeletal muscle lysate in the 1) presence and 2) absence of immunizing peptide using this antibody at 2.0 ug/ml.

Product Discontinued

ATG16L1 Antibody Summary

• Immunogen: A portion of amino acids 25-75 of human APG16L was used as the immunogen.
• Predicted Species: Rat (91%), Canine (95%), Equine (95%), Monkey (100%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG16L1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0-3.0 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee and monkey,95% homologous in dog, horse and mouse,91% homologous incow and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for ATG16L1 Antibody

• APG16 autophagy 16-like (S. cerevisiae)
• APG16L beta
• APG16LFLJ10828
• APG16-like 1
• ATG16 autophagy related 16-like (S. cerevisiae)
• ATG16 autophagy related 16-like 1 (S. cerevisiae)
• ATG16A
• ATG16L
• autophagy-related protein 16-1
• FLJ00045
• FLJ10035
• FLJ22677
• IBD10
• WD repeat domain 30
• WDR30

Background

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is essential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane. There are 5 isoforms of this protein (in human), with theoretical molecular weights ranging from ~19-68 kDa.

Mutations in the ATG16L1 proitein have been associated with Crohn's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG16L1