ATG16L1 Antibody

Product Discontinued

ATG16L1 Antibody Summary

• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide from near the center of human ATG16. The immunogen is located within amino acids 190 - 240 of ATG16.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG16L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry
  • Western Blot 0.25-0.5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATG16L1 Antibody

• APG16 autophagy 16-like (S. cerevisiae)
• APG16L beta
• APG16LFLJ10828
• APG16-like 1
• ATG16 autophagy related 16-like (S. cerevisiae)
• ATG16 autophagy related 16-like 1 (S. cerevisiae)
• ATG16A
• ATG16L
• autophagy-related protein 16-1
• FLJ00045
• FLJ10035
• FLJ22677
• IBD10
• WD repeat domain 30
• WDR30

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ATG16L1 Antibody (NBP1-76863). (Showing 1 - 1 of 1 FAQ).

1. Does this antibody cross react with ATG16L2?
   • We have not yet tested for cross reactivity with ATG16L2. Based on the immunogen sequence, there is little similarity between the sequences in the region that NBP1-76863 was made, so I would not expect this product to detect ATG16L2. The immunogen sequence is found between amino acids 201-250 of NP_110430.

Bioinformatics

• Gene Symbol: ATG16L1
• Uniprot:
  • NP_110430(Human)