ATF5 Antibody (9Z3M0)

Western Blot: ATF5 Antibody (9Z3M0) [NBP3-16221] - Western blot analysis of extracts of various cell lines, using ATF5 Rabbit mAb (NBP3-16221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry-Paraffin: ATF5 Antibody (9Z3M0) [NBP3-16221] - Immunohistochemistry of paraffin-embedded human placenta using ATF5 Rabbit mAb (NBP3-16221) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 9Z3M0
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ATF5 Antibody (9Z3M0) Summary

• Description: Novus Biologicals Rabbit ATF5 Antibody (9Z3M0) (NBP3-16221) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 183-282 of human ATF5 (Q9Y2D1). PQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATF5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:1000 - 1:6000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ATF5 Antibody (9Z3M0)

• activating transcription factor 5FLJ34666
• ATFXHMFN0395
• cAMP-dependent transcription factor ATF-5
• cyclic AMP-dependent transcription factor ATF-5
• Transcription factor ATFx

Background

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF5