Human ATF4 ELISA Kit (Colorimetric)

ELISA: Human ATF4 ELISA Kit (Colorimetric) [NBP2-69883] - Samples were spiked with high concentrations of Human ATF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human ATF4 ELISA Kit (Colorimetric) [NBP2-69883] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human ATF4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ATF4 in samples. No significant cross-reactivity or interference between Human ATF4 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.13%
• Intra-Assay: CV% < 5.14%
• Spike Recovery: 86-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ATF4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ATF4 ELISA Kit (Colorimetric)

• activating transcription factor 4 (tax-responsive enhancer element B67)
• Activating transcription factor 4
• ATF4
• cAMP-dependent transcription factor ATF-4
• cAMP-responsive element-binding protein 2
• CREB-2DNA-binding protein TAXREB67
• cyclic AMP-dependent transcription factor ATF-4
• Cyclic AMP-responsive element-binding protein 2
• TAXREB67
• TAXREB67CREB2cAMP response element-binding protein 2
• Tax-responsive enhancer element-binding protein 67
• TXREB

Background

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ATF4