Human ATF4 ELISA Kit (Colorimetric)

ELISA: Human ATF4 ELISA Kit (Colorimetric) [NBP2-69883] - Samples were spiked with high concentrations of Human ATF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human ATF4 ELISA Kit (Colorimetric) [NBP2-69883] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human ATF4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ATF4 in samples. No significant cross-reactivity or interference between Human ATF4 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.13%
• Intra-Assay: CV% < 5.14%
• Spike Recovery: 86-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ATF4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ATF4 ELISA Kit (Colorimetric)

• activating transcription factor 4 (tax-responsive enhancer element B67)
• Activating transcription factor 4
• ATF4
• cAMP-dependent transcription factor ATF-4
• cAMP-responsive element-binding protein 2
• CREB-2DNA-binding protein TAXREB67
• cyclic AMP-dependent transcription factor ATF-4
• Cyclic AMP-responsive element-binding protein 2
• TAXREB67
• TAXREB67CREB2cAMP response element-binding protein 2
• Tax-responsive enhancer element-binding protein 67
• TXREB

Background

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for ATF4 ELISA Kit (NBP2-69883). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ATF4