ATF4 Antibody (739441) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 739441
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

ATF4 Antibody (739441) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human ATF4
  Met1-Pro351
  Accession # P18848
• Specificity: Detects human ATF4 in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATF4
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for ATF4 Antibody (739441) [CoraFluor™ 1]

• activating transcription factor 4 (tax-responsive enhancer element B67)
• Activating transcription factor 4
• ATF4
• cAMP-dependent transcription factor ATF-4
• cAMP-responsive element-binding protein 2
• CREB-2DNA-binding protein TAXREB67
• cyclic AMP-dependent transcription factor ATF-4
• Cyclic AMP-responsive element-binding protein 2
• TAXREB67
• TAXREB67CREB2cAMP response element-binding protein 2
• Tax-responsive enhancer element-binding protein 67
• TXREB

Background

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF4