Ataxin 1 Antibody

Western Blot: Ataxin 1 Antibody [NBP1-28734] - Detection of Human Ataxin-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Ataxin-1 antibody NBP1-28734 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Ataxin-1 was also immunoprecipitated by rabbit anti-Ataxin-1 antibody NBP1-28690, which recognizes an upstream epitope. For blotting immunoprecipitated Ataxin-1, the ReliaBLOT (RESTRICTED) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Product Discontinued

Ataxin 1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 766 and 816 of human ataxin-1 using the numbering given in entry NP_000323.2 (GeneID 6310).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Rabbit (100%), Guinea Pig (100%), Bovine (100%), Canine (100%), Chinese Hamster (100%), Equine (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATXN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody is useful for western blot and immunoprecipitation.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque, African elephant and Northern white-cheeked gibbon.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ataxin 1 Antibody

• ataxin 1
• ataxin 1)
• ataxin-1
• ATX1spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant
• SCA1D6S504E
• Spinocerebellar ataxia type 1 protein

Background

Defects in Ataxin-1 are the cause of spinocerebellar ataxia type 1 (SCA1), also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 is caused by expansion of a CAG repeat in the coding region of the ataxin-1 gene. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. Ataxin-1 binds RNA in vitro and may be involved in RNA metabolism. [Uniprot]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATXN1
• Entrez:
  • 6310(Human)
• Uniprot:
  • NP_000323.2(Human)