ATAD3A Antibody - Azide and BSA Free

Western Blot: ATAD3A Antibody [H00055210-B01P] - Analysis of ATAD3A expression in human spleen.

Immunocytochemistry/Immunofluorescence: ATAD3A Antibody [H00055210-B01P] - ATAD3A in human primary fibroblasts. Mitochondrial localization. PFA 4% fixed. Primary incubation 10 ug/mL O/N 4C. Secondary: Donkey anti-mouse Alexafluor 555 1:500 1h RT.

Western Blot: ATAD3A Antibody [H00055210-B01P] - Analysis of ATAD3A expression in HeLa.

Western Blot: ATAD3A Antibody [H00055210-B01P] - Analysis of ATAD3A expression in transfected 293T cell line by ATAD3A polyclonal antibody. Lane 1: ATAD3A transfected lysate(64.46 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ATAD3A Antibody [H00055210-B01P] - Analysis of purified antibody to ATAD3A on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATAD3A Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ATAD3A Antibody - Azide and BSA Free (H00055210-B01P) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ATAD3A (AAH33109.1, 1 a.a. - 586 a.a.) full-length human protein. MSWLFGINKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKDALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQQLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVEAEARARAKAERENADIIREQIRLKAAEHRQTVLESIRTAGTLFGEGFRAFVTDWDKVTATVAGLTLLAVGVYSAKNATLVAGRFIEARLGKPSLVRETSRITVLEALRHPIQVSRRLLSRPQDALEGVVLSPSLEARVRDIAIATRNTKKNRSLYRNILMYGPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMGREGVTAMHKLFDWANTSRRGLLLFVDEADAFLRKRATEKISEDLRATLNAFLYRTGQHSNKFMLVLASNQPEQFDWAINDRINEMVHFDLPGQEERERLVRMYFDKYVLKPATEGKQRLKLAQFDYGRKCSEVARLTEGMSGREIAQLAVSWQATAYASEDGVLTEAMMDTRVQDAVQQHQQKMCWLKAEGPGRGDEPSPS
• Specificity: ATAD3A - ATPase family, AAA domain containing 3A,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: ATAD3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in Western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATAD3A Antibody - Azide and BSA Free

• ATPase family AAA domain-containing protein 3A
• ATPase family, AAA domain containing 3A
• FLJ10709
• FLJ35514

Background

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al., 2007 [PubMed 17210950]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for ATAD3A Antibody (H00055210-B01P) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/08/2020

Summary

• Application: Western Blot
• Sample Tested: Human primary fibroblast
• Species: Human
• Lot: J2151

Comments

• Comments: Abnova ab ATAD3A. TGX 4-15% gel. Denaturalized and reductive conditions. Blocking Milk 5% in TBST (0.1% Tween). Primary ab incubation 1:1000 in Milk 5% in TBST (0.1% Tweeen) ON 4ºC. Secondary 1:5000 Goat anti-mouse alexa fluor 647 1h RT.

reviewed by: Verified Customer ICC Human 11/08/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: Human primary fibroblast
• Species: Human
• Lot: J2151

Comments

• Comments: Not checked if it detects only ATAD3A. Human primary fibroblasts PFA 4% fixed. Blocking 1h DG 10% in PBST (0.1% Triton). Primary incubation 10 ug/ml O/N 4ºC. Secondary: Donkey anti-mouse Alexafluor 555 1:500 1h RT.

Bioinformatics

• Gene Symbol: ATAD3A
• Entrez:
  • 55210(Human)
• Uniprot:
  • AAH33109.1(Human)