Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free

Western Blot: Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free [NBP2-70215] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Asparagine synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti

Immunocytochemistry/Immunofluorescence: Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free [NBP2-70215] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Asparagine synthetase.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: OTI1A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ASNS(NP_597680) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASNS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 64.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free

• ASNS
• ASNSD
• asparagine synthetase (glutamine-hydrolyzing)
• asparagine synthetase [glutamine-hydrolyzing]
• asparagine synthetase
• Cell cycle control protein TS11
• EC 6.3.5.4
• Glutamine Dependent Asparagine Synthetase
• Glutamine-dependent asparagine synthetase
• TS11 Cell Cycle Control Protein
• TS11

Background

Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASNS