ASCL1/Mash1 Antibody (290622)

Product Discontinued

ASCL1/Mash1 Antibody (290622) Summary

• Immunogen: E. coli-derived recombinant mouse ASCL1/Mash1
  His74-Asp173 (Gln160Glu)
  Accession # Q53WV9
• Specificity: Detects mouse ASCL1/Mash1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ASCL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASCL1/Mash1 Antibody (290622)

• achaete-scute complex (Drosophila) homolog-like 1
• achaete-scute complex homolog 1 (Drosophila)
• achaete-scute complex-like 1 (Drosophila)
• achaete-scute homolog 1
• ASCL1
• ASH1
• ASH-1
• ASH1MASH1
• BHLHA46
• bHLHa46achaete-scute complex-like 1
• Class A basic helix-loop-helix protein 46
• HASH1
• HASH1achaete scute protein
• Mash1

Background

ASCL1 (achaete-scute complex-like 1), also known as Mash1, is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation. Within the region used as an immunogen, mouse ASCL1 shares 97% amino acid sequence identity with human ASCL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASCL1/Mash1 Antibody (MAB2567)(2)

Publications using MAB2567

• N Tiwari, A Pataskar, S Péron, S Thakurela, SK Sahu, M Figueres-O, N Marichal, L López-Masc, VK Tiwari, B Berninger Stage-Specific Transcription Factors Drive Astrogliogenesis by Remodeling Gene Regulatory Landscapes Cell Stem Cell, 2018-10-04;23(4):557-571.e8. 2018-10-04 [PMID: 30290178] (ICC, Western Blot, Mouse)
• Ikeda K, Ookawara S, Sato S, Ando Z, Kageyama R, Kawakami K Six1 is essential for early neurogenesis in the development of olfactory epithelium. Dev. Biol., 2007-08-16;311(1):53-68. 2007-08-16 [PMID: 17880938] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: ASCL1
• Uniprot:
  • Q53WV9()