ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702) [Alexa Fluor™ Plus 647]

• Clone: 511702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse ASAHL is observed and no cross-reactivity with recombinant human ASAH2 or recombinant mouse ASAH2 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702) [Alexa Fluor™ Plus 647]

• Acid ceramidase-like protein
• ASAHL
• ASAH-like protein
• EC 3.5.1.-
• NAAA
• N-acylethanolamine acid amidase
• Nacylethanolaminehydrolyzing Acid Amidase
• N-acylethanolamine-hydrolyzing acid amidase
• N-acylsphingosine amidohydrolase (acid ceramidase)-like
• N-acylsphingosine amidohydrolase-like
• PLT

Background

The human NAAA gene encodes N-acylethanolamine-hydrolyzing acid amidase, also known as ASAH-like protein (ASAHL), a fatty acid amidase with maximal activity at acidic pH (1). ASAHL hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N‑palmitoylethanolamine (2). ASAHL is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). ASAHL is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). ASAHL can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

1. Hong, S.B. et al. (1999) Genomics 62:232.
2. Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
3. Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.