ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702) [Alexa Fluor® 750]

• Applications: WB, IP
• Clone: 511702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702) [Alexa Fluor® 750] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
  Ser29-Lys359 (Val151Ile)
  Accession # Q02083
• Specificity: Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse ASAHL is observed and no cross-reactivity with recombinant human ASAH2 or recombinant mo
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

The human NAAA gene encodes N-acylethanolamine-hydrolyzing acid amidase, also known as ASAH-like protein (ASAHL), a fatty acid amidase with maximal activity at acidic pH (1). ASAHL hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N‑palmitoylethanolamine (2). ASAHL is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). ASAHL is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). ASAHL can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.