ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N‑acylsphingosine Amidohydrolase-2
  Thr34-Thr756
  Accession # Q9JHE3
• Specificity: Detects mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human ASAH2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ASAH2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

• ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
• ASAH2
• BCDase
• HNAC1
• LCDase
• Nacylsphingosine Amidohydrolase2
• NCDase
• N-CDase

Background

The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, also known as neutral ceramidase. Neutral ceramidase is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). A major physiological function of neutral ceramidase is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

1. Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
2. Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
3. Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody (AF3558)(1)

Publication using AF3558

• Fui G. Goh, Anna M. Piccinini, Thomas Krausgruber, Irina A. Udalova, Kim S. Midwood Transcriptional Regulation of the Endogenous Danger Signal Tenascin-C: A Novel Autocrine Loop in Inflammation The Journal of Immunology 2010-03-01 [PMID: 20107185]

Bioinformatics

• Gene Symbol: ASAH2
• Uniprot:
  • Q9JHE3()