ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [Alexa Fluor® 532]

• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N‑acylsphingosine Amidohydrolase-2
  Thr34-Thr756
  Accession # Q9JHE3
• Specificity: Detects mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human ASAH2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, also known as neutral ceramidase. Neutral ceramidase is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). A major physiological function of neutral ceramidase is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.