ARVCF Antibody (5D2) - Azide and BSA Free

Western Blot: ARVCF Antibody (5D2) [H00000421-M01] - ARVCF monoclonal antibody (M01), clone 5D2 Analysis of ARVCF expression in A-431.

Immunohistochemistry-Paraffin: ARVCF Antibody (5D2) [H00000421-M01] - Analysis of monoclonal antibody to ARVCF on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.

• Reactivity: Hu, Xp
• Applications: WB, ELISA, IHC, IP
• Clone: 5D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARVCF Antibody (5D2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ARVCF (NP_001661, 863 a.a. ~ 962 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LSPGGFDDSTLPLVDKSLEGEKTGSRDVIPMDALGPDGYSTVDRRERRPRGASSAGEASEKEPLKLDPSRKAPPPGPSRPAVRLVDAVGDAKPQPVDSWV
• Specificity: ARVCF - armadillo repeat gene deletes in velocardiofacial syndrome
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARVCF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARVCF Antibody (5D2) - Azide and BSA Free

• armadillo repeat gene deleted in velocardiofacial syndrome
• armadillo repeat protein deleted in velo-cardio-facial syndrome
• FLJ35345

Background

Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome (ARVCF) is a member of the catenin family which play an important role in the formation of adherens junction complexes, which are thought to facilitate communication between the inside and outside environments of a cell. ARVCF gene was isolated in the search for the genetic defect responsible for the autosomal dominant Velo-Cardio-Facial syndrome (VCFS) a relatively common human disorder with phenotypic features including cleft palate, conotruncal heart defects and facial dysmorphology. ARVCF gene encodes a protein containing two motifs, a coiled coil domain in the N-terminus and a 10 armadillo repeat sequence in the midregion. Since these sequences can facilitate protein-protein interactions ARVCF is thought to function in a protein complex. In addition, ARVCF contains a predicted nuclear-targeting sequence suggesting that it may have a function as a nuclear protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARVCF Antibody (H00000421-M01)(2)

Publications using H00000421-M01

• Suzuki N, Idogawa M, Tange S et al. p53-induced ARVCF modulates the splicing landscape and supports the tumor suppressive function of p53. Oncogene. 2019-12-11 [PMID: 31827232]
• Cho K, Vaught TG, Ji H et al. Xenopus Kazrin interacts with ARVCF-catenin, spectrin and p190B RhoGAP, and modulates RhoA activity and epithelial integrity. J Cell Sci;123(Pt 23):4128-44. 2010-12-01 [PMID: 21062899]

Bioinformatics

• Gene Symbol: ARVCF
• Entrez:
  • 421(Human)
• OMIM:
  • 602269(Human)
• Uniprot:
  • NP_001661(Human)