Artemis Overexpression Lysate (Native)

Images

 
Western Blot: Artemis Overexpression Lysate (Adult Normal) [NBL1-09743] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Artemis.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Artemis Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203407 and based on accession number NM_022487. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae) (DCLRE1C), transcript variant b, mRNA.
Gene
DCLRE1C

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
65.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Artemis

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Artemis Overexpression Lysate (Native)

  • ASCID
  • DCLREC1C
  • DNA cross-link repair 1C protein
  • DNA cross-link repair 1C
  • EC 3.1
  • FLJ36438
  • hSNM1C
  • protein artemis
  • Protein A-SCID
  • PSO2 homolog
  • RS-SCID
  • severe combined immunodeficiency, type a (Athabascan)
  • SNM1 homolog C
  • SNM1CS. cerevisiae)
  • SNM1-like protein

Background

This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq]. Transcript Variant: This variant (b), also called variant 3, lacks an exon in the 3' coding region, compared to variant a. It encodes isoform b which has a shorter and distinct N-terminus, compared to variant a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Artemis Lysate (NBL1-09743) (0)

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Bioinformatics

Gene Symbol DCLRE1C