ARIH2 Antibody

Western Blot: ARIH2 Antibody [NB100-1163] - Analysis of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARIH2 Antibody Summary

• Description: Novus Biologicals Goat ARIH2 Antibody (NB100-1163) is a polyclonal antibody validated for use in WB, ELISA and IP. Anti-ARIH2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EQRRRTLLKDFHDT corresponding to C-Terminus according to NP_006312.1.
• Localization: Nuclear
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARIH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: IF: An anonymous customer has reported nuclear staining in DAPI. IP: work well is noted. WB: Approx. 60 kDa band observed in nuclear lysates of Hela and K562 (calculated MW of 57.8 kDa band according to NP_006312.1). An anonymous customer has reported an approx. 60 kDa band in lysates of both human HeLa and mouse muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down (calculated MW of 57.8 kDa band according to NP_006312.1).
• Control:

  ARIH2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARIH2 Antibody

• ARI-2
• ariadne (Drosophila) homolog 2
• ariadne homolog 2 (Drosophila)
• E3 ubiquitin-protein ligase ARIH2
• EC 6.3.2.-
• FLJ10938
• FLJ33921
• Protein ariadne-2 homolog
• Triad1 protein
• TRIAD1ARI2all-trans retinoic acid inducible RING finger

Background

Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARIH2 Antibody (NB100-1163)(1)

Publication using NB100-1163

• van der Reijden BA, Erpelinck-Verschueren CA, Lowenberg B, Jansen JH. TRIADs: a new class of proteins with a novel cysteine-rich signature. Protein Sci 1999-07-01 [PMID: 10422847]

Bioinformatics

• Gene Symbol: ARIH2
• Entrez:
  • 10425(Human)
  • 23807(Mouse)
• Uniprot:
  • O95376(Human)