ARID1A Antibody (CL3595) - BSA Free

Western Blot: ARID1A Antibody (CL3595) [NBP2-61623] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)

Immunocytochemistry/Immunofluorescence: ARID1A Antibody (CL3595) [NBP2-61623] - Staining of A-431 cells using the Anti-ARID1A monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: ARID1A Antibody (CL3595) [NBP2-61623] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ARID1A Antibody (CL3595) [NBP2-61623] - Staining of human breast cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: ARID1A Antibody (CL3595) [NBP2-61623] - Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ARID1A Antibody (CL3595) [NBP2-61623] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ARID1A Antibody (CL3595) [NBP2-61623] - Staining of human liver shows weak nuclear positivity in hepatocytes.

Immunohistochemistry-Paraffin: ARID1A Antibody (CL3595) [NBP2-61623] - Staining of human prostate cancer shows strong nuclear positivity in tumor cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: CL3595
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ARID1A Antibody (CL3595) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from O14497, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (95%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARID1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  ARID1A Recombinant Protein Antigen (NBP2-61623PEP)

Reactivity Notes

Expected species cross reactivity based on sequence identity: Rat (88%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for ARID1A Antibody (CL3595) - BSA Free

• ARID domain-containing protein 1A
• AT rich interactive domain 1A (SWI- like)
• AT rich interactive domain 1A (SWI-like)
• AT-rich interactive domain-containing protein 1A
• B120SWI-like protein
• BAF250a
• BAF250SWI/SNF complex protein p270
• BM029
• brain protein 120
• BRG1-associated factor 250
• BRG1-associated factor 250a
• C10rf4
• C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1
• chromatin remodeling factor p250
• hELD
• hOSA1
• matrix associated, actin dependent regulator of chromatin
• Osa homolog 1
• OSA1 nuclear protein
• OSA1
• P270
• SMARCF1
• subfamily f, member 1

Background

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARID1A