ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01] - Analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M01), clone 3A12-B5.Lane 1: ARHGEF5 transfected lysate(60.1 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: ARHGEF5 Antibody (3A12-B5) [H00007984-M01] - Detection limit for recombinant GST tagged ARHGEF5 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 3A12-B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA
• Specificity: ARHGEF5 - Rho guanine nucleotide exchange factor (GEF) 5
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGEF5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

• ephexin-3
• GEF5
• Guanine nucleotide regulatory protein TIM
• Oncogene TIM
• p60 TIM
• P60
• Rho guanine nucleotide exchange factor (GEF) 5
• rho guanine nucleotide exchange factor 5
• TIM1
• TIMDKFZp686N1969
• Transforming immortalized mammary oncogene

Background

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARHGEF5 Antibody (H00007984-M01)(1)

Publication using H00007984-M01

• Mendrzyk F, Korshunov A, Benner A et al. Identification of gains on 1q and epidermal growth factor receptor overexpression as independent prognostic markers in intracranial ependymoma. Clin Cancer Res. 2006-04-01 [PMID: 16609018]

Bioinformatics

• Gene Symbol: ARHGEF5
• Entrez:
  • 7984(Human)
• OMIM:
  • 600888(Human)
• Uniprot:
  • AAH14555(Human)