ARHGEF11 Antibody

Western Blot: ARHGEF11 Antibody [NBP1-06553] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-06553 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PDZ-RhoGEF was also immunoprecipitated by NBP1-06552, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: ARHGEF11 Antibody [NBP1-06553] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PDZ-RhoGEF used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ARHGEF11 Antibody Summary

• Description: Novus Biologicals Rabbit ARHGEF11 Antibody (NBP1-06553) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-ARHGEF11 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1472 and 1522 of human PDZ-RhoGEF using the numbering given in entry NP_055599.1 (GeneID 9826).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGEF11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in ICC/IF reported in scientific literature (PMID: 30219706).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARHGEF11 Antibody

• DKFZp667F1223
• glutamate transporter EAAT4-associated protein 48
• GTRAP48
• KIAA0380rho guanine nucleotide exchange factor 11
• PDZ-RHOGEF
• Rho guanine exchange factor (GEF) 11
• Rho guanine nucleotide exchange factor (GEF) 11
• RhoA-specific guanine nucleotide exchange factor
• RhoGEF glutamate transport modulator

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARHGEF11 Antibody (NBP1-06553)(2)

Publications using NBP1-06553

• Ding Z, Dong Z, Yang Y Et Al. The guanine nucleotide exchange factor, LARG, and RhoC play a role in glioblastoma cell invasion and resistance Am. J. Pathol. 2020-07-18 [PMID: 32693062]
• Ding Z, Dhruv H, Kwiatkowska-Piwowarczyk A et al. PDZ-RhoGEF Is a Signaling Effector for TROY-Induced Glioblastoma Cell Invasion and Survival. Neoplasia 2018-10-01 [PMID: 30219706] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ARHGEF11
• Entrez:
  • 9826(Human)
• Uniprot:
  • O15085(Human)