Argininosuccinate Lyase Antibody (OTI3D4)

Western Blot: Argininosuccinate Lyase Antibody (3D4) [NBP2-46548] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Argininosuccinate Lyase.

Immunohistochemistry: Argininosuccinate Lyase Antibody (3D4) [NBP2-46548] - Analysis of Carcinoma of Human liver tissue.

Western Blot: Argininosuccinate Lyase Antibody (3D4) [NBP2-46548] - Analysis of HT29 cell lysate (35ug).

Immunohistochemistry: Argininosuccinate Lyase Antibody (3D4) [NBP2-46548] - Analysis of Human liver tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Argininosuccinate Lyase Antibody (OTI3D4) Summary

• Description: Novus Biologicals Mouse Argininosuccinate Lyase Antibody (OTI3D4) (NBP2-46548) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ASL (NP_001020114) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 51.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Argininosuccinate Lyase Antibody (OTI3D4)

• argininosuccinase
• argininosuccinate lyase
• arginosuccinase
• ASAL
• EC 4.3.2.1

Background

Argininosuccinate Lyase encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASL