ARFGAP2 Antibody

Western Blot: ARFGAP2 Antibody [NBP2-15441] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ARFGAP2 Antibody [NBP2-15441] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: ARFGAP2 Antibody [NBP2-15441] - Paraffin-embedded colon ca, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ARFGAP2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ARFGAP2. The exact sequence is proprietary.
• Localization: Cytoplasm; Golgi apparatus membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARFGAP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ARFGAP2 Antibody

• ADP-ribosylation factor GTPase activating protein 2
• ADP-ribosylation factor GTPase-activating protein 2
• ARF GAP 2
• FLJ14576
• FLJ26000
• IRZ
• putative protein product of Nbla10535
• Zfp289
• Zinc finger protein 289
• zinc finger protein 289, ID1 regulated
• ZNF289GTPase-activating protein ZNF289

Background

ADP-ribosylation factor GTPase activating protein 2 (ArfGAP2) is a GTPase activating protein that interacts with the GTPase ADP-ribosylation factor (Arf) in Golgi membranes and vesicles. Although they are differentially regulated, ArfGAP2 along with ArfGAP1 and ArfGAP3 has been shown to play an overlapping role in COPI-mediated transport and in the maintenance of Golgi organization. ArfGAP2 is also known as zinc finger protein 289 or ZNF289.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ARFGAP2
• Uniprot:
  • Q8N6H7(Human)