ARF3 Antibody - Azide and BSA Free

Western Blot: ARF3 Antibody [NBP3-12541] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Raji Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:20 kDa

Immunoprecipitation: ARF3 Antibody [NBP3-12541] - Lane A:0.5 mg HeLa Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg Raji Whole Cell LysateLane D:0.5 mg MCF7 Whole Cell Lysate4 uL anti- ARF3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti- ARF3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 19kDaObserved band size :19 kDa

Immunocytochemistry/Immunofluorescence: ARF3 Antibody [NBP3-12541] - Staining of ARF3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12541 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARF3 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human ARF3 (Uniprot#: P61204; Met1-Lys181).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARF3
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunoprecipitation 1-5ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ARF3 Antibody - Azide and BSA Free

• ADP-ribosylation factor 3
• small GTP binding protein

Background

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARF3