ARF3 Antibody

Images

 
Western Blot: ARF3 Antibody [NBP1-31004] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE ADP-ribosylation factor 3 antibody, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ARF3 Antibody [NBP1-31004] - Immunofluorescence analysis of methanol-fixed HeLa, using ARF3 antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ARF3 Antibody [NBP1-31004] - Sample: Paraffin-embedded mouse fore brain. ARF3 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: ARF3 Antibody [NBP1-31004] - Sample: Paraffin-embedded human colon carcinoma. ARF3 antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Sh, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ARF3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF3. The exact sequence is proprietary.
Localization
Golgi apparatus
Specificity
This product has been tested only for its reactivity with ARF3, however the sequence homology of the immunogen indicates a possible cross reactivity with ARF1, ARF5 and ARF6
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Sheep (100%), Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ARF3 Lysate (NBP2-65545)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for ARF3 Antibody

  • ADP-ribosylation factor 3
  • small GTP binding protein

Background

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARF3
Entrez
Uniprot