ARAP1 Antibody (ARAP1-2) - BSA Free

Immunocytochemistry/Immunofluorescence: ARAP1 Antibody (ARAP1-2) [NB110-68801] - Colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0, 30 or 60 and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: ARAP1-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ARAP1 Antibody (ARAP1-2) - BSA Free Summary

• Description: Novus Biologicals Mouse ARAP1 Antibody (ARAP1-2) - BSA Free (NB110-68801) is a monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1
• Specificity: The ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARAP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 2 ug/ml
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for ARAP1 Antibody (ARAP1-2) - BSA Free

• arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
• ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
• centaurin, delta 2
• Centaurin-delta-2
• CENTD2ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containingprotein 1
• cnt-d2
• KIAA0782centaurin-delta-2

Background

ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARAP1
• Entrez:
  • 116985(Human)