Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free

Staining of human duodenum shows strong positivity in plasma in blood vessels.

Staining of human colon shows moderate to strong positivity in plasma in blood vessels.

Staining of human skin shows no positivity in squamous epithelial cells as expected.

Analysis in human plasma.

Staining of human urinary bladder shows moderate positivity in plasma in blood vessels.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0171
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free (NBP2-14785) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of O14791, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free

• APOL
• APO-L
• APOL1
• APO-L1
• APO-LI
• APOL-I
• Apolipoprotein L
• apolipoprotein L, 1
• Apolipoprotein L1
• Apolipoprotein L-I
• FSGS4

Background

Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein L gene family encodes six highly homologous proteins designated apoL-I to -VI, which are associated with large high density type lipoproteins (HDL). The human apoL family maps to chromosome 22q12.1- 13.1 within a 127,000-bp region . apoL has been characterized as a pancreas specific, 383-amino acid, 42 kDa protein that contains a 12-amino acid secretory signal peptide. The apoL genes have TATA-less promoters and contain putative sterol regulatory elements, suggesting that transcription of these genes may be coordinated with that of the low density lipoprotein receptor and genes in pathways involving the synthesis of triglycerides and cholesterol. apoL homologs can undergo 10 fold changes in expression during atherosclerotic changes in vascular endothelial cells, which includes the inflammatory reaction of atherosclerotic lesions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOL1