Rat Apolipoprotein H/ApoH ELISA Kit (Colorimetric)

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Rat Apolipoprotein H/ApoH ELISA Kit (Colorimetric) [NBP2-77026] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
28 ng/mL - 1800 ng/mL
Sensitivity
7 ng/mL

Order Details

Rat Apolipoprotein H/ApoH ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat Apolipoprotein H/ApoH. No significant cross-reactivity or interference between Rat Apolipoprotein H/ApoH and analogues was observed.
Standard Curve Range
28 ng/mL - 1800 ng/mL
Sensitivity
7 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Spike Recovery
89 - 110%
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
APOH

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay Plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction Manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Rat Apolipoprotein H/ApoH ELISA Kit (Colorimetric)

  • Activated protein C-binding protein
  • Anticardiolipin cofactor
  • APC inhibitor
  • APOH
  • apo-H
  • apolipoprotein H (beta-2-glycoprotein I)
  • Apolipoprotein H
  • B2G1
  • B2GP1
  • B2GPI
  • Beta
  • beta(2)GPI
  • beta-2-glycoprotein 1
  • Beta-2-glycoprotein I
  • BG

Background

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol APOH