Rat Apolipoprotein H/ApoH ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 1-5 hours |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Rat Apolipoprotein H/ApoH. No significant cross-reactivity or interference between Rat Apolipoprotein H/ApoH and analogues was observed. |
Standard Curve Range |
28 ng/mL - 1800 ng/mL |
Sensitivity |
7 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Spike Recovery |
89 - 110% |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
APOH |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay Plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction Manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Rat Apolipoprotein H/ApoH ELISA Kit (Colorimetric)
Background
Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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