Apolipoprotein E/ApoE Antibody - BSA Free

Western Blot: Apolipoprotein E/ApoE Antibody [NBP2-41216] - Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 ug/mL.

Immunohistochemistry: Apolipoprotein E/ApoE Antibody - BSA Free [NBP2-41216] - Immunohistochemistry of Apolipoprotein E/ApoE inhuman liver tissue with Apolipoprotein E/ApoE antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Apolipoprotein E/ApoE Antibody - BSA Free [NBP2-41216] - Immunofluorescence of APO E in mouse brain tissue with APO E Antibody at 20 ug/mL. Green: APO E antibody Red: Phylloidin staining Blue: DAPI staining

Immunohistochemistry: Apolipoprotein E/ApoE Antibody - BSA Free [NBP2-41216] - Immunohistochemistry of APO E in mouse brain tissue with APO E Antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Apolipoprotein E/ApoE Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Apolipoprotein E/ApoE Antibody - BSA Free (NBP2-41216) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E. The immunogen is located within amino acids 220 - 270 of APO-E. Amino Acid Squence: ARMEEMGSRTRDRLDEVKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOE
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 - 2 ug/mL
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Apolipoprotein E/ApoE Antibody - BSA Free

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOE