Apolipoprotein C-II/ApoC2 Antibody (2554B) [Unconjugated]

Apolipoprotein C‑II/ApoC2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF‑7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (Catalog # MAB44971) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Apolipoprotein C‑II/ApoC2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (Catalog # MAB44971) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Human Apolipoprotein C‑II/ApoC2 protein was serially diluted 2-fold and captured by Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (MAB4497) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (Catalog # MAB44971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: ELISA, IHC, ICC/IF
• Clone: 2554B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Apolipoprotein C-II/ApoC2 Antibody (2554B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived human Apolipoprotein C‑II/ApoC2
  Thr23-Glu101
  Accession # P02655
• Specificity: Detects human Apolipoprotein C‑II/ApoC2 in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 0.3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein C-II/ApoC2 Antibody (2554B) [Unconjugated]

• APC2
• APOC2
• Apo-CII
• apoC-II
• Apolipoprotein C2
• Apolipoprotein CII
• Apolipoprotein C-II
• MGC75082

Background

Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P02655()