Apolipoprotein A5 Antibody (4H8H8E2) - BSA Free

• Reactivity: Hu
• Applications: ELISA
• Clone: 4H8H8E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Apolipoprotein A5 Antibody (4H8H8E2) - BSA Free Summary

• Immunogen: Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOA5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
• Application Notes: This Apolipoprotein A5 antibody is useful for ELISA.
• Control:

  Apolipoprotein A5 Overexpression Lysate

Reactivity Notes

Immunogen sequence has 83% identity to Bovine, 79% identity to rat and 78% identity to mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Apolipoprotein A5 Antibody (4H8H8E2) - BSA Free

• APOAV
• apo-AV
• APOA-V
• Apolipoprotein A5
• apolipoprotein A-V
• FLJ97995
• MGC126836
• RAP3MGC126838
• Regeneration-associated protein 3

Background

Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein A5 Antibody (NB400-138)(1)

Publication using NB400-138

• Pennacchio, LA et al. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing. Science;294(5540):169-73. 2001-10-05 [PMID: 11588264]

Bioinformatics

• Gene Symbol: APOA5
• Entrez:
  • 116519(Human)
• Uniprot:
  • Q6Q788(Human)