Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Western Blot:[NBP3-30393]-Detection of human ApoAI by western blot. Samples: Whole cell lysate from Hep-G2, MCF10A, SR, Malme-3M, and PC-3 cells prepared using NETN lysis buffer. Antibody: Affinity Purified Rabbit anti-Human Apo AI [A03R] Antibody used for WB at 1 µg/ml. Secondary: HRP-conjugated Goat anti-Rabbit IgG Heavy and Light Chain Cross-Adsorbed Antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Apolipoprotein A-I/ApoA1 Antibody - BSA Free Summary

• Immunogen: Human Apolipoprotein AI
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, pH 7.2
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

• Alp-1
• APOA1
• Apo-AI
• apoA-I
• Apolipoprotein A1
• Apolipoprotein AI
• Apolipoprotein A-I
• Brp-14
• Ltw-1
• Lvtw-1
• MGC117399
• Sep-1
• Sep-2

Background

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOA1