APIP Antibody - BSA Free

Western Blot: APIP Antibody [NB100-56383] - Analysis of APIP 2 in human muscle using this antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: APIP Antibody [NB100-56383] - Formalin-fixed, paraffin-embedded human kidney tissue probed with this antibody at 5 ug/ml.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

APIP Antibody - BSA Free Summary

• Immunogen: The antibody was developed against a full-length His-tagged recombinant APIP protein (NP_057041).
• Specificity: This polyclonal can be used for detection of APIP and APIP2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APIP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  APIP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for APIP Antibody - BSA Free

• APAF1 interacting protein
• APAF1-interacting protein
• APIP2
• CGI29
• CGI-29
• dJ179L10.2
• EC 4.2.1.109
• Mmrp19
• MTRu-1-P dehydratase
• probable methylthioribulose-1-phosphate dehydratase

Background

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Recently, Cho et al (4) have identified a new Apaf-1 Interacting Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APIP
• Entrez:
  • 51074(Human)
• Uniprot:
  • Q96GX9(Human)