EI2BL Antibody (OTI9H7)

Western Blot: EI2BL Antibody (9H7) [NBP1-47857] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EI2BL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EI2BL.

Immunocytochemistry/Immunofluorescence: EI2BL Antibody (9H7) [NBP1-47857] Staining of COS7 cells transiently transfected by pCMV6-ENTRY EI2BL.

Immunohistochemistry-Paraffin: EI2BL Antibody (9H7) [NBP1-47857] - Staining of paraffin-embedded Human endometrium tissue using anti-EI2BL mouse monoclonal antibody.

Flow Cytometry: EI2BL Antibody (9H7) [NBP1-47857] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EI2BL antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: EI2BL Antibody (9H7) [NBP1-47857] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EI2BL mouse monoclonal antibody.

Immunohistochemistry-Paraffin: EI2BL Antibody (9H7) [NBP1-47857] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EI2BL mouse monoclonal antibody.

Immunohistochemistry-Paraffin: EI2BL Antibody (9H7) [NBP1-47857] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EI2BL mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI9H7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.18 mg/ml

EI2BL Antibody (OTI9H7) Summary

• Description: Novus Biologicals Mouse EI2BL Antibody (OTI9H7) (NBP1-47857) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human MRI1 (NP_115661) produced in HEK293T cell.
• Specificity: This antibody is specific for Homo sapiens methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (MRI1), transcript variant 1.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 39.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.18 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EI2BL Antibody (OTI9H7)

• EC 5.3.1.23
• M1Pi
• Mediator of RhoA-dependent invasion
• methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
• methylthioribose-1-phosphate isomerase
• MGC3207
• MRDI
• MTNA
• MTR-1-P isomerase
• S-methyl-5-thioribose-1-phosphate isomerase
• Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
• Ypr118w

Background

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) (By similarity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRI1
• Entrez:
  • 84245(Human)