API5 Antibody - BSA Free

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Western Blot: API5 Antibody - BSA Free [NBP3-48128] - HepG2 cells were subjected to SDS PAGE followed by western blot with (API5 Antibody) at dilution of 1:300
Immunohistochemistry: API5 Antibody - BSA Free [NBP3-48128] - Immunohistochemistry of paraffin-embedded human pancreas tissue slide using (API5 Antibody) at dilution of 1:50
Immunocytochemistry/ Immunofluorescence: API5 Antibody - BSA Free [NBP3-48128] - Immunofluorescent analysis of 10% Formaldehyde fixed HepG2 cells using (API5 antibody) at dilution of 1:50 and FITC-conjugated Goat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

API5 Antibody - BSA Free Summary

Immunogen
apoptosis inhibitor 5. (Uniprot# Q9BZZ5)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
API5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
Application Notes
Theoretical Molecular Weight is 50-55 kDa

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% Glycerol, pH (7.3)
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for API5 Antibody - BSA Free

  • AAC-11API5L1
  • AAC11fibroblast growth factor 2-interacting factor 2
  • Antiapoptosis clone 11 protein
  • API-5
  • apoptosis inhibitor 5
  • Cell migration-inducing gene 8 protein
  • Fibroblast growth factor 2-interacting factor
  • FIF
  • migration-inducing protein MIG8
  • Protein XAGL

Background

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol API5