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AP2 alpha Antibody

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Dot Blot: AP2 alpha Antibody [NB100-56332] - Analysis of AP-2 in MCF-7 cell lysate with this antibody. A protein band of an approximate molecular weight of 55 kDa is detected.
Dot Blot: AP2 alpha Antibody [NB100-56332] - Analysis of AP-2 in MCF-7 cell lysate with this antibody. A protein band of an approximate molecular weight of 55 kDa is detected.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Pm, Sh, Xp, ChSpecies Glossary
Applications WB, DB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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AP2 alpha Antibody Summary

Immunogen
A portion of amino acids 100-150 of human AP-2 was used as the immunogen.
Specificity
The amino acid sequence used as immunogen has 100% identity in human, mouse, rat, bovine, dog, monkey, sheep and Xenopus, and 93% in chicken.
Predicted Species
Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TFAP2A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Dot Blot
Control
MCF-7 Whole Cell Lysate
AP2 alpha Knockout HeLa Cell Lysate

Reactivity Notes

93% sequence identity with chicken protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for AP2 alpha Antibody

  • Activating enhancer-binding protein 2-alpha
  • Activator protein 2
  • AP-2 transcription factor
  • AP2-alpha
  • AP2TFAP-2alpha
  • AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
  • BOFS
  • FLJ51761
  • TFAP2
  • transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
  • transcription factor AP-2-alpha

Background

The members of the AP-2 family of Transcription factors are developmentally regulated and have functions in the regulation of many genes governing growth and differentiation. AP-2 transcription factors have been shown to act both as transcriptional activators and repressors in a promoter-specific manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
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Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control. [1] protein standard (red), [2] HeLa cells in serum free media. [3] HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media. [4] rat cortical neurons. [5] rat cortical neurons treated with membrane depolarization buffer for 5hrs. Multiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten. The single band at 37 kDa represents GAPDH protein.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
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Western Blot: FosB/G0S3 Antibody (83B1138) [NB100-56530] - Analysis of FosB in HeLa cell lysate where bands are observed at ~38 kDa representing FosB and at ~35 kDa representing delta FosB.
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Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-JunD(S255) antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: JunB/AP-1 Antibody [NBP1-89544] - Analysis in human cell lines MCF-7 and HEK293 using anti-JUNB antibody. Corresponding JUNB RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
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Control Lysate(s)

MCF-7 Whole Cell Lysate
AP2 alpha Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol TFAP2A
Entrez
Uniprot
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