Antidiuretic Hormone Antibody - BSA Free

• Reactivity: All-Multi
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 5.0 mg/ml

Antidiuretic Hormone Antibody - BSA Free Summary

• Description: Hypothalamus and posterior lobe of the pituitary gland are reliable positive control tissues for IHC experiments.
• Immunogen: Synthetic Antidiuretic Hormone conjugated to bovine thyroglobulin
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AVP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000 - 1:20000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Broad species cross-reactivity expected from sequence.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 5.0 mg/ml
• Purity: Protein G purified

Alternate Names for Antidiuretic Hormone Antibody - BSA Free

• ADH
• arginine vasopressin
• ARVP
• Vasopressin

Background

This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20. [provided by RefSeq, Nov 2015]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Antidiuretic Hormone Antibody (NB100-65214)(2)

Publications using NB100-65214

• Li J, Zhang X, He Q Et al. Pancreatic neuroendocrine tumor producing vasopressin: A case report Medicine 2021-10-08 [PMID: 34622867] (IF/IHC, Human)
• Zhang Y, Xu Y, Ding H et al. Prenatal exposure of female mice to per uorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 production Chemosphere 2020-10-27 [PMID: 33131727] (IF/IHC, Mouse)

FAQs for Antidiuretic Hormone Antibody (NB100-65214). (Showing 1 - 1 of 1 FAQs).

1. We ordered the Arg-vasopressin Antibody (NB100-65214) (0.05 ml) last week and also received it. I was wondering whether you have any idea about the working dilution since there is nothing indicated in the datasheet?
   • The recommended starting dilution for IHC is 1:100-1:200 and for IF we recommend 1:50-1:250. These are just starting dilutions and should be optimized.

Bioinformatics

• Gene Symbol: AVP
• Entrez:
  • 280728(Bovine)
  • 551(Human)
  • 11998(Mouse)
  • 24221(Rat)
• Uniprot:
  • P01185()
  • PO1185(Human)