Annexin A7 Antibody - BSA Free

Western Blot: Annexin A7 Antibody [NBP1-31442] - A. 30 ug 293T whole cell extractB. 30 ug A431 whole cell extractC. 30 ug HeLa whole cell extractD. 30 ug HepG2 whole cell extract10 % SDS-PAGE Annexin VII antibody dilution: 1:1000

Immunocytochemistry/Immunofluorescence: Annexin A7 Antibody [NBP1-31442] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: ANXA7 protein stained by Annexin VII antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: Annexin A7 Antibody [NBP1-31442] - Paraffin-embedded human gastric carcinoma dilution: 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Annexin A7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Annexin A7 Antibody - BSA Free (NBP1-31442) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Annexin A7. The exact sequence is proprietary.
• Predicted Species: Mouse (94%), Rat (94%), Rhesus Macaque (98%), Bovine (93%), Chimpanzee (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANXA7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Annexin A7 Antibody - BSA Free

• Annexin A7
• ANX7
• ANXA7
• SNX
• Synexin

Background

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ANXA7